1. 缺血性腦中風與神經保護劑：

   我們的實驗室致力於研究缺血性腦中風的生理機制及其治療方法。缺血性腦中風是由於腦部血液供應中斷而引起的一種常見疾病，我們的研究重點在於開發新型神經保護劑，以減少腦中風所造成的損害。通過深入研究神經細胞的生理反應和腦部病變的機制，我們希望能夠找到更有效的治療方法，以改善腦中風患者的生活品質。

1. Ischemic Stroke and Neuroprotective Agents:

   Our laboratory is dedicated to researching the mechanisms and treatment of ischemic stroke. Ischemic stroke, caused by the interruption of blood flow to the brain, is a common neurological disorder. We focus on developing novel neuroprotective agents to minimize the damage caused by stroke. Through in-depth studies of neuronal responses and brain pathology, we aim to identify more effective treatment strategies to improve the quality of life for stroke patients.  ADDIN EN.CITE.DATA  ADDIN EN.CITE.DATA [1-35]

1.       Tai S-H, Chao L-C, Huang S-Y, et al. Nicotinamide Deteriorates Post-Stroke Immunodepression Following Cerebral Ischemia–Reperfusion Injury in Mice. Biomedicines. 2023;11(8):2145.

2.       Tai SH, Lee WT, Lee AC, et al. Therapeutic window for YC‑1 following glutamate‑induced neuronal damage and transient focal cerebral ischemia. Mol Med Rep. 2018 May;17(5):6490-6496.

3.       Lin YW, Chen TY, Hung CY, et al. Melatonin protects brain against ischemia/reperfusion injury by attenuating endoplasmic reticulum stress. Int J Mol Med. 2018 Jul;42(1):182-192.

4.       Lee WT, Tai SH, Lin YW, et al. YC‑1 reduces inflammatory responses by inhibiting nuclear factor‑κB translocation in mice subjected to transient focal cerebral ischemia. Mol Med Rep. 2018 Aug;18(2):2043-2051.

5.       Huang SY, Tai SH, Chang CC, et al. Magnolol protects against ischemic-reperfusion brain damage following oxygen-glucose deprivation and transient focal cerebral ischemia. Int J Mol Med. 2018 Apr;41(4):2252-2262.

6.       Huang SY, Chang CH, Hung HY, et al. Neuroanatomical and electrophysiological recovery in the contralateral intact cortex following transient focal cerebral ischemia in rats. Neurol Res. 2018 Feb;40(2):130-138.

7.       Yu-Wen Lin S-HT, Chih-Hao Tien, Sheng-Yang Huang, Che-Chao Chang, Tian-Shung Wu, Wei-Sheng Juan, Hung-Yi Chen,  E-JL. Cinnamophilin Inhibits Neutrophilic Respiratory Burst and Protects Against Ischemia-Reperfusion Brain Damage [Research Article]. Pharmaceutica Analytica Acta. 2013 Nov 21;4(10):6.

8.       Shih HC, Hwang TL, Chen HC, et al. Honokiol dimers and magnolol derivatives with new carbon skeletons from the roots of Magnolia officinalis and their inhibitory effects on superoxide anion generation and elastase release. PLoS One. 2013;8(5):e59502.

9.       Lee WT, Lin MH, Lee EJ, et al. Magnolol reduces glutamate-induced neuronal excitotoxicity and protects against permanent focal cerebral ischemia up to 4 hours. PLoS One. 2012;7(7):e39952.

10.     Juan WS, Lin HW, Chen YH, et al. Optimal Percoll concentration facilitates flow cytometric analysis for annexin V/propidium iodine-stained ischemic brain tissues. Cytometry A. 2012 May;81(5):400-8.

11.     Huang GJ, Bhaskar Reddy MV, Kuo PC, et al. A concise synthesis of viscolin, and its anti-inflammatory effects through the suppression of iNOS, COX-2, ERK phosphorylation and proinflammatory cytokines expressions. Eur J Med Chem. 2012 Feb;48:371-8.

12.     Chen TY, Lin MH, Lee WT, et al. Nicotinamide inhibits nuclear factor-kappa B translocation after transient focal cerebral ischemia. Crit Care Med. 2012 Feb;40(2):532-7.

13.     Chang CC, Tien CH, Lee EJ, et al. Melatonin inhibits matrix metalloproteinase-9 (MMP-9) activation in the lipopolysaccharide (LPS)-stimulated RAW 264.7 and BV2 cells and a mouse model of meningitis. J Pineal Res. 2012 Sep;53(2):188-97.

14.     Tai SH, Hung YC, Lee EJ, et al. Melatonin protects against transient focal cerebral ischemia in both reproductively active and estrogen-deficient female rats: the impact of circulating estrogen on its hormetic dose-response. J Pineal Res. 2011 Apr;50(3):292-303.

15.     Chen TY, Tai SH, Lee EJ, et al. Cinnamophilin offers prolonged neuroprotection against gray and white matter damage and improves functional and electrophysiological outcomes after transient focal cerebral ischemia. Crit Care Med. 2011 May;39(5):1130-7.

16.     Tai SH, Chen HY, Lee EJ, et al. Melatonin inhibits postischemic matrix metalloproteinase-9 (MMP-9) activation via dual modulation of plasminogen/plasmin system and endogenous MMP inhibitor in mice subjected to transient focal cerebral ischemia. J Pineal Res. 2010 Nov;49(4):332-41.

17.     Hung YC, Chou YS, Chang CH, et al. Early reperfusion improves the recovery of contralateral electrophysiological diaschisis following focal cerebral ischemia in rats. Neurol Res. 2010 Oct;32(8):828-34.

18.     Lin HW, Lee EJ. Effects of melatonin in experimental stroke models in acute, sub-acute, and chronic stages. Neuropsychiatr Dis Treat. 2009;5:157-62.

19.     Lee EJ, Hung YC, Chen HY, et al. Delayed treatment with carboxy-PTIO permits a 4-h therapeutic window of opportunity and prevents against ischemia-induced energy depletion following permanent focal cerebral ischemia in mice. Neurochem Res. 2009 Jun;34(6):1157-66.

20.     Lee EJ, Chen HY, Hung YC, et al. Therapeutic window for cinnamophilin following oxygen-glucose deprivation and transient focal cerebral ischemia. Exp Neurol. 2009 May;217(1):74-83.

21.     Chen HY, Tai SH, Lee EJ. Magnesium treatment and spontaneous mild hypothermia after transient focal cerebral ischemia in the rat. Brain Res Bull. 2009 May 29;79(3-4):224-5.

22.     Hung YC, Chen TY, Lee EJ, et al. Melatonin decreases matrix metalloproteinase-9 activation and expression and attenuates reperfusion-induced hemorrhage following transient focal cerebral ischemia in rats. J Pineal Res. 2008 Nov;45(4):459-67.

23.     Cheng CY, Ho TY, Lee EJ, et al. Ferulic acid reduces cerebral infarct through its antioxidative and anti-inflammatory effects following transient focal cerebral ischemia in rats. Am J Chin Med. 2008;36(6):1105-19.

24.     Lee MY, Kuan YH, Chen HY, et al. Intravenous administration of melatonin reduces the intracerebral cellular inflammatory response following transient focal cerebral ischemia in rats. J Pineal Res. 2007 Apr;42(3):297-309.

25.     Lee EJ, Wu TS, Chang GL, et al. Delayed treatment with nicotinamide inhibits brain energy depletion, improves cerebral microperfusion, reduces brain infarct volume, but does not alter neurobehavioral outcome following permanent focal cerebral ischemia in Sprague Dawley rats. Curr Neurovasc Res. 2006 Aug;3(3):203-13.

26.     Fang SY, Tseng CC, Yang YL, et al. Nitric oxide scavenger carboxy-PTIO reduces infarct volume following permanent focal ischemia. Acta Anaesthesiol Taiwan. 2006 Sep;44(3):141-6.

27.     Chen TY, Lee MY, Chen HY, et al. Melatonin attenuates the postischemic increase in blood-brain barrier permeability and decreases hemorrhagic transformation of tissue-plasminogen activator therapy following ischemic stroke in mice. J Pineal Res. 2006 Apr;40(3):242-50.

28.     Chen HY, Chen TY, Lee MY, et al. Melatonin decreases neurovascular oxidative/nitrosative damage and protects against early increases in the blood-brain barrier permeability after transient focal cerebral ischemia in mice. J Pineal Res. 2006 Sep;41(2):175-82.

29.     Lee EJ, Lee MY, Chen HY, et al. Melatonin attenuates gray and white matter damage in a mouse model of transient focal cerebral ischemia. J Pineal Res. 2005 Jan;38(1):42-52.

30.     Lee EJ, Lee MY, Chang GL, et al. Delayed treatment with magnesium: reduction of brain infarction and improvement of electrophysiological recovery following transient focal cerebral ischemia in rats. J Neurosurg. 2005 Jun;102(6):1085-93.

31.     Lee EJ, Chen HY, Lee MY, et al. Cinnamophilin reduces oxidative damage and protects against transient focal cerebral ischemia in mice. Free Radic Biol Med. 2005 Aug 15;39(4):495-510.

32.     Lee EJ, Wu TS, Lee MY, et al. Delayed treatment with melatonin enhances electrophysiological recovery following transient focal cerebral ischemia in rats. J Pineal Res. 2004 Jan;36(1):33-42.

33.     Lee EJ, Chen HY, Wu TS, et al. Acute administration of Ginkgo biloba extract (EGb 761) affords neuroprotection against permanent and transient focal cerebral ischemia in Sprague-Dawley rats. J Neurosci Res. 2002 Jun 1;68(5):636-45.

34.     Mokudai T, Ayoub IA, Sakakibara Y, et al. Delayed treatment with nicotinamide (Vitamin B(3)) improves neurological outcome and reduces infarct volume after transient focal cerebral ischemia in Wistar rats. Stroke. 2000 Jul;31(7):1679-85.

35.     Lee EJ, Ayoub IA, Harris FB, et al. Mexiletine and magnesium independently, but not combined, protect against permanent focal cerebral ischemia in Wistar rats. J Neurosci Res. 1999 Nov 1;58(3):442-8.

 

2. 缺血性腦中風與神經可塑性：

   神經再塑是指神經系統對外部刺激和損傷的適應能力，特別是在經過缺血性腦中風後的神經損傷，需要通過神經再塑來恢復神經原本的功能。我們相信透過了解神經再塑的過程，可以開發出更有效的康復治療策略。我們的研究將重點放在探索神經元神經軸的生長和突觸重建過程，並評估不同治療方法對神經可塑性的影響，以期為腦中風康復治療提供新的思路和方法。

Ischemic Stroke and Neuroplasticity:

   Neuroplasticity refers to the adaptability of the nervous system to external stimuli and injuries, particularly in the context of neuro damage following ischemic stroke, where neural function restoration relies heavily on neuroplasticity. We believe that understanding the process of neuroplasticity can lead to the development of more effective rehabilitation strategies. Our research will focus on exploring the processes of neurite growth and synaptic reconstruction, and evaluating the impact of different treatment methods on neuroplasticity, aiming to provide new insights and approaches for stroke recovery therapy. [1-5]

1.       Lee A-C, Tai S-H, Chen Y-Y, et al. Effect of prothymosin α on neuroplasticity following cerebral ischemia‑reperfusion injury. Mol Med Rep. 2024 2024/04/01;29(4):59.

2.       Tai SH, Huang SY, Chao LC, et al. Lithium upregulates growth-associated protein-43 (GAP-43) and postsynaptic density-95 (PSD-95) in cultured neurons exposed to oxygen-glucose deprivation and improves electrophysiological outcomes in rats subjected to transient focal cerebral ischemia following a long-term recovery period. Neurol Res. 2022 Mar 29:1-9.

3.       Tai S-H, Chao L-C, Huang T-Y, et al. Short-term lithium treatment protects the brain against ischemia–reperfusion injury by enhancing the neuroplasticity of cortical neurons. Neurological Research. 2021 Feb;44(2):128-138.

4.       Juan WS, Huang SY, Chang CC, et al. Melatonin improves neuroplasticity by upregulating the growth-associated protein-43 (GAP-43) and NMDAR postsynaptic density-95 (PSD-95) proteins in cultured neurons exposed to glutamate excitotoxicity and in rats subjected to transient focal cerebral ischemia even during a long-term recovery period. J Pineal Res. 2014 Mar;56(2):213-23.

5.       Chen HY, Hung YC, Chen TY, et al. Melatonin improves presynaptic protein, SNAP-25, expression and dendritic spine density and enhances functional and electrophysiological recovery following transient focal cerebral ischemia in rats. J Pineal Res. 2009 Oct;47(3):260-70.

 

3. 神經腫瘤相關研究：

   神經腫瘤相關研究，旨在深入了解神經腫瘤的發生機制和治療方法。神經腫瘤是一種常見的神經系統疾病，對患者的生活造成了嚴重影響。我們的研究將集中於腫瘤細胞的生長調控機制、腫瘤微環境的影響以及新型治療方法的開發，旨在提高神經腫瘤患者的治療效果和生存率。

3. Neurotumor-related Research:

   Our laboratory is dedicated to neurotumor-related research, aiming to understand the mechanisms of tumorigenesis and treatment strategies. Neurotumors are common neurological disorders that significantly impact patients' lives. Our research focuses on the regulation of tumor cell growth, the influence of the tumor microenvironment, and the development of novel therapies to improve treatment outcomes and survival rates for neurotumor patients. [1-6]

1.       Xiong A, Li J, Xiong R, et al. Inhibition of HIF-1α-AQP4 axis ameliorates brain edema and neurological functional deficits in a rat controlled cortical injury (CCI) model. Scientific Reports. 2022 2022/02/17;12(1):2701.

2.       Tai SH, Lin YW, Huang TY, et al. Cinnamophilin enhances temozolomide-induced cytotoxicity against malignant glioma: the roles of ROS and cell cycle arrest. Transl Cancer Res. 2021 Sep;10(9):3906-3920.

3.       Tai SH, Lin YW, Chen TY, et al. Comparison of fresh/frozen isolation method in DNA preparation of meningiomas for flow cytometry. Int J Clin Exp Pathol. 2017;10(11):11057-11062.

4.       Suzuki N, Arimitsu N, Shimizu J, et al. Neuronal Cell Sheets of Cortical Motor Neuron Phenotype Derived from Human iPSCs. Cell Transplant. 2017 Aug;26(8):1355-1364.

5.       Lin YW, Tai SH, Chang CC, et al. Application of flow cytometry for evaluating clinical prognosis and histopathological grade of human glioma. Neurol Res. 2016 Jul;38(7):625-33.

6.       Lin YW, Tai SH, Huang YH, et al. The application of flow cytometry for evaluating biological aggressiveness of intracranial meningiomas. Cytometry B Clin Cytom. 2015 Sep-Oct;88(5):312-9.

4. 神經外科臨床研究：

   神經外科臨床研究，旨在改善神經外科手術的治療效果和患者的生活品質。神經外科手術是治療神經系統疾病的重要手段，但也存在著一定的風險和挑戰。我們的研究將關注手術技術的改進、手術後康復治療策略的優化，以及手術後併發症的預防和治療，以期為神經外科患者提供更安全、更有效的治療方案。

4. Clinical Research in Neurosurgery:

   Our laboratory conducts clinical research in neurosurgery to improve surgical outcomes and patient quality of life. Neurosurgery is a critical treatment for neurological disorders, but it comes with risks and challenges. Our research focuses on improving surgical techniques, optimizing postoperative rehabilitation strategies, and preventing and treating postoperative complications. We aim to provide safer and more effective treatment options for neurosurgical patients.  ADDIN EN.CITE.DATA [1-18]

1.       Chen YY, Chao LC, Tai SH, et al. Spontaneous resolution of a symptomatic cerebellopontine angle arachnoid cyst in adult: A case report and literature review. Kaohsiung J Med Sci. 2024 Jan;40(1):105-106.

2.       Chen YY, Chuang MT, Tai SH, et al. Choroid plexus papilloma at the root entry zone causing hemifacial spasm. Kaohsiung J Med Sci. 2023 Aug 24.

3.       Huang CC, Tai SH, Lai CH, et al. Comparison of an intravertebral reduction device and percutaneous vertebroplasty for anatomical reduction with single-level vertebral compression fractures. Formos J Surg. 2020 May-Jun;53(3):101-108.

4.       Chen YY, Chao LC, Fang JJ, et al. 3D-Customized Guiding Template for Posterior Fixation in Complex Atlantoaxial Instability-Preliminary Experiences of National Cheng Kung University Hospital. J Neurol Surg Rep. 2020 Jan;81(1):e20-e27.

5.       Chang CC, Tien CH, Tai SH, et al. High-grade glioma in a patient with breast cancer. Asian J Surg. 2014 Jul;37(3):162-6.

6.       Tsai CH, Lee EJ, Liu YS, et al. Isolated oculomotor nerve palsy caused by diffuse large B cell lymphoma. Acta Neurol Belg. 2013 Mar;113(1):103-4.

7.       Lu CH, Chang KC, Lee EJ, et al. Intracranial Rosai-Dorfman disease with unusual transcranial extension. J Neuroimaging. 2012 Jul;22(3):312-5.

8.       Juan WS, Tai SH, Hung YC, et al. Multiple tenting techniques improve dead space obliteration in the surgical treatment for patients with giant calcified chronic subdural hematoma. Acta Neurochir (Wien). 2012 Apr;154(4):707-10; discussion 710.

9.       Huang CY, Lee PH, Lin SH, et al. Remote cerebellar hemorrhage following supratentorial craniotomy. Neurol Res. 2012 Jun;34(5):422-9.

10.     Huang YC, Wei KC, Chang CH, et al. A retrospective survey of patients with malignant gliomas treated in the neuro-oncological care system under the Universal National Health Insurance program in Taiwan. J Clin Neurosci. 2011 Jun;18(6):784-8.

11.     Tai SH, Hung YC, Chen JC, et al. A patient with two episodes of thoracic spinal cord compression caused by primary lymphoma and metastatic carcinoma of the prostate, 11 years apart. Kaohsiung J Med Sci. 2010 Sep;26(9):506-10.

12.     Hung YC, Lee EJ, Chen HY, et al. Effects of propofol sedation during the early postoperative period in hemorrhagic stroke patients. Acta Anaesthesiol Taiwan. 2009 Sep;47(3):128-33.

13.     Huang DW, Tai SH, Hung YC, et al. A rare occipital condyle fracture in a patient with a minor head injury. Kaohsiung J Med Sci. 2009 Jun;25(6):342-6.

14.     Huang CY, Hung YC, Tai SH, et al. Cerebellar hemorrhage after multiple manual pumping tests of a ventriculoperitoneal shunt: a case report. Kaohsiung J Med Sci. 2009 Jan;25(1):29-33.

15.     Lee MY, Lee EJ. Giant cell tumor of the petrous temporal bone with direct invasion into the middle ear. J Craniofac Surg. 2006 Jul;17(4):797-800.

16.     Lee EJ, Lee MY, Shyr MH, et al. Adjuvant bupivacaine scalp block facilitates stabilization of hemodynamics in patients undergoing craniotomy with general anesthesia: a preliminary report. J Clin Anesth. 2006 Nov;18(7):490-4.

17.     Lee EJ, Chang CH, Wang LC, et al. Two primary brain tumors, meningioma and glioblastoma multiforme, in opposite hemispheres of the same patient. J Clin Neurosci. 2002 Sep;9(5):589-91.

18.     Lee EJ, Hung YC, Wang LC, et al. Factors influencing the functional outcome of patients with acute epidural hematomas: analysis of 200 patients undergoing surgery. J Trauma. 1998 Nov;45(5):946-52.